Herbal Science Research - herbal

Human hair growth enhancement in vitro by green tea epigallocatechin-3-gallate (EGCG).

Site Editor — Wed, 26 Sep 2007 18:49:37 -0700

Human hair growth enhancement in vitro by green tea epigallocatechin-3-gallate (EGCG).: Phytomedicine. 2007 Aug;14(7-8):551-5 Authors: Kwon OS, Han JH, Yoo HG, Chung JH, Cho KH, Eun HC, Kim KH

Green tea is a popular worldwide beverage, and its potential beneficial effects such as anti-cancer and anti-oxidant properties are believed to be mediated by epigallocatechin-3-gallate (EGCG), a major constituent of polyphenols. Recently, it was reported that EGCG might be useful in the prevention or treatment of androgenetic alopecia by selectively inhibiting 5alpha-reductase activity. However, no report has been issued to date on the effect of EGCG on human hair growth. This study was undertaken to measure the effect of EGCG on hair growth in vitro and to investigate its effect on human dermal papilla cells (DPCs) in vivo and in vitro. EGCG promoted hair growth in hair follicles ex vivo culture and the proliferation of cultured DPCs. The growth stimulation of DPCs by EGCG in vitro may be mediated through the upregulations of phosphorylated Erk and Akt and by an increase in the ratio of Bcl-2/Bax ratio. Similar results were also obtained in in vivo dermal papillae of human scalps. Thus, we suggest that EGCG stimulates human hair growth through these dual proliferative and anti-apoptotic effects on DPCs.

PMID: 17092697 [PubMed - indexed for MEDLINE]

Mobile PubMed Feeds

Site Editor — Fri, 20 Jul 2007 19:08:26 -0700

Most of the traffic I see run through this site is based around existing, single-source RSS feeds. The most popular of which is the PubMed Herbal RSS feed. Some folks have tapped into the multi-term taxonomy searching and RSS generation, but most users seem to keep things simple.

Any RSS feed coming out of this site can be read on pretty much any device that's RSS-savvy. The problem is that doing this usually requires a mobile RSS reader application, should you want to stay informed, and stay mobile. Flash and Java options appear to be cumbersome on most mobile devices, even if these technologies are supported.

So, using Feedburner's XML-HTML parsing toolset, I've added a couple of very simple html pages at mobile.herbalscienceresearch.com that display the latest abstracts and full text articles being indexed by PubMed.

The first is a slightly modified version of the above mentioned PubMed abstract feed. I've filtered out some of the more advanced phytochemistry results, yielding a slightly less reductionistic version of the original. There's also a new mobile version of the PubMed Herbal Full Text feed, providing a way to stay current with PubMed's full text articles of a herbal nature.

The pages have been formatted to render nicely for my shiny new iPhone, but should display well on any html-capable device with a viewport equivalent of 320px. Of course, they render in a browser nicely too, making them a nice option for non-mobile dial-up users who don't want to load images, javascript, etc.

Speaking of javascript, a cool search page is in the works. Meanwhile, you can view the mobile abstract feed at mobile.herbalscienceresearch.com and tap into the mobile full text feed here. mobile.herbalscienceresearch.com/fulltext.

[...] The need to examine and investigate traditional remedies for pharmacotherapy of animal and zoonotic diseases.

Site Editor — Fri, 13 Jul 2007 18:03:00 -0700

Newer directions for plant drug research: The need to examine and investigate traditional remedies for pharmacotherapy of animal and zoonotic diseases.: Afr J Health Sci. 1998 Feb;5(1):12-4 Authors: Kofi-Tsekpo MW, Kioy DW

Traditional remedies for animal diseases have not been investigated as much as those for human diseases. Yet, there is a wealth of knowledge available to be tapped from the remedies used in the treatment of animal and zoonotic diseases. The indigenous knowledge available could be rationalized in the modern pharmaceutical context and applied directly to field use where appropriate. Furthermore, it is well known that many drugs have been developed initially through their use in animals because the ethical issues are easier to handle. It is worth examining some the indigenous medicinal plants that are used for both human and animal disease treatment, and determine the practical and cost-effective ways of exploiting the values of these plants.

PMID: 17580988 [PubMed - in process]

The berberis story: Berberis vulgaris in therapeutics.

Site Editor — Mon, 11 Jun 2007 06:47:46 -0700

The berberis story: Berberis vulgaris in therapeutics.: Pak J Pharm Sci. 2007 Jan;20(1):83-92 Authors: Arayne MS, Sultana N, Bahadur SS

Barberry has played a prominent role in herbal healing for more than 2,500 years. Berberis vulgaris is a common garden bush, native to Europe and the British Isles, naturalized in North America, seems to have history as old as human race. Anthropologists believe in a ritual practice or sacred object, especially by Native Americans that it works as a supernatural power or as preventive or remedy of illness. It is a deciduous shrub having yellow flowers and scarlet colored fruit in the form of berries. Twenty two alkaloids have been reported so far from root, stem leaves and fruit of this plant, which are of medicinal importance. As a herbal remedy it has no match in serving human race since ancient times. It is the most widely used drug in Homeopathic system of medicine for kidney pain and for removal of kidney stones. In this article, we present countless blessings of nature encountered through this herb which are worthy of recording.

Advances in the Use of Milk Thistle (Silybum marianum).

Site Editor — Mon, 11 Jun 2007 05:32:46 -0700

Advances in the Use of Milk Thistle (Silybum marianum).: Integr Cancer Ther. 2007 Jun;6(2):104-9 Authors: Post-White J, Ladas EJ, Kelly KM

Milk thistle (Silybum marianum) is an herbal supplement used to treat liver and biliary disorders. Silymarin, a mixture of flavanoid complexes, is the active component that protects liver and kidney cells from toxic effects of drugs, including chemotherapy. Although milk thistle has not significantly altered the course of chronic liver disease, it has reduced liver enzyme levels and demonstrated anti-inflammatory and T cell-modulating effects. There is strong preclinical evidence for silymarin's hepatoprotective and anticarcinogenic effects, including inhibition of cancer cell growth in human prostate, skin, breast, and cervical cells. Milk thistle is considered safe and well-tolerated, with gastrointestinal upset, a mild laxative effect, and rare allergic reaction being the only adverse events reported when taken within the recommended dose range. More clinical trials of rigorous methodology, using standardized and well-defined products and dosages, are needed to evaluate the potential of silymarin against liver toxicity, chronic liver disease, and human cancers.

An examination of the prescription and dispensing of medicines by Western herbal therapists: a national survey in Australia.

Site Editor — Fri, 25 May 2007 02:35:11 -0700

An examination of the prescription and dispensing of medicines by Western herbal therapists: a national survey in Australia.: Complement Ther Med. 2007 Mar;15(1):13-20 Authors: Casey MG, Adams J, Sibbritt D

OBJECTIVE: To describe the patterns of prescription and dispensing of herbal medicines employed by Western herbal practitioners in Australia. DESIGN: A national postal survey sent to all full members of the National Herbalist Association of Australia (NHAA). SETTING: Nationwide clinics of professional herbal practitioners in Australia. MAIN OUTCOME MEASURES: Measures of medicinal use were collected including aspects of prescription, formulation, dispensing and preparation forms of medicines used. The practitioners were asked if they dispensed their own medicines, about the form of herbal preparations used, aspects of formulation and dosages. RESULTS: The predominant prescription by most Western herbal practitioners in Australia is individualised herbal formulae rather than the use of single herbs. Traditional preparations of herbals such as teas and powders are used but liquid herbal extracts are most commonly dispensed. These liquid medicines are in the form of highly concentrated fluid extracts in ratios of 1:1 or 1:2, herb material to liquid. Pre-formulated tablets or capsules made by herbal manufacturers are being incorporated into modern practice but most prescriptions are individualised liquid formulae prepared and dispensed, from the clinic dispensary, after patient consultation. In addition to internal prescriptions, topical creams, pessaries, douches, gargles, eyebaths and poultices or washes are still incorporated into treatments. Although there is variation in used dosage ranges, the predominant system is that of pharmacologically active doses of highly concentrated fluid extracts. CONCLUSION: The professional prescription of Western herbal medicines is different to commercial usage. As opposed to pre-formulated tablets, or use of single herbals, the vast majority of Western herbalists in Australia construct individualised herbal formulations for their patients after consultation. The preferred form of administration of these prescriptions is as highly concentrated liquid herbal extracts in pharmacologically active doses.

[History of opium poppy and morphine]

Site Editor — Fri, 11 May 2007 15:44:42 -0700

[History of opium poppy and morphine]: Dan Medicinhist Arbog. 2005;33:171-84 Authors: Norn S, Kruse PR, Kruse E

Opium has been known for millennia to relieve pain and its use for surgical analgesia has been recorded for several centuries. The Sumerian clay tablet (about 2100 BC) is considered to be the world's oldest recorded list of medical prescriptions. It is believed by some scholars that the opium poppy is referred to on the tablet. Some objects from the ancient Greek Minoan culture may also suggest the knowledge of the poppy. A goddess from about 1500 BC shows her hair adorned probably with poppy-capsules and her closed eyes disclose sedation. Also juglets probably imitating the poppy-capsules were found in that period in both Cyprus and Egypt. The first authentic reference to the milky juice of the poppy we find by Theophrastus at the beginning of the third century BC. In the first century the opium poppy and opium was known by Dioscorides, Pliny and Celsus and later on by Galen. Celsus suggests the use of opium before surgery and Dioscorides recommended patients should take mandrake (contains scopolamine and atropine) mixed with wine, before limb amputation. The Arabic physicians used opium very extensively and about 1000 AD it was recommended by Avicenna especially in diarrhoea and diseases of the eye. Polypharmacy, including a mixture of nonsensical medications were often used. Fortunately for both patients and physicians many of the preparations contained opium. The goal was a panacea for all diseases. A famous and expensive panacea was theriaca containing up to sixty drugs including opium. Simplified preparations of opium such as tinctura opii were used up to about 2000 in Denmark. In the early 1800s sciences developed and Sertürner isolated morphine from opium and was the founder of alkaloid research. A more safe and standardized effect was obtained by the pure opium. Several morphine-like drugs have been synthesized to minimize adverse effects and abuse potential. Opioid receptors were identified and characterized in binding assays and their localization examined. However, the complexity of the system including interaction with several neurons and transmitters indicate the goal of nonaddictive opiates to be elusive. Combination therapy, innovative delivery systems and long-acting formulations may improve clinical utility.

Simon Mills video on Google Video

Site Editor — Fri, 09 Feb 2007 19:03:25 -0800

This video has been posted over on Google Video . It is of the esteemed Simon Mills, MCPP, FNIMH, MA, lecturing at Schumacher College in Devon, England.

The video is titled,"Hot and Cold: Crucial Aspects of Herbalism".

Medicinal plants used in Kirklareli Province (Turkey).

Site Editor — Wed, 31 Jan 2007 19:04:35 -0800

Medicinal plants used in Kirklareli Province (Turkey).: J Ethnopharmacol. 2006 Dec 12; Authors: Kültür S

In this paper, 126 traditional medicinal plants from Kirklareli Province in Turkey have been reported. One hundred and twenty six plant species belonging to 54 families and among them 100 species were wild and 26 species were cultivated plants. Most used families were Rosaceae, Labiatae, Compositae and the most used plants were Cotinus coggyria, Sambucus ebulus, Achillea millefolium subsp. pannonica, Hypericum perforatum, Matricaria chamomilla var. recutita, Melissa officinalis subsp. officinalis, Juglans regia, Thymus longicaulis subsp. longicaulis var. subisophyllus, Malva sylvestris, Urtica dioica, Plantago lanceolata, Rosa canina, Ecballium elaterium, Artemisia absinthium, Viscum album subsp. album, Papaver rhoeas, Helleborus orientalis, Cydonia oblonga, Prunus spinosa subsp. dasyphylla, Rubus discolor, Sorbus domestica. A total of 143 medicinal uses were obtained. The traditional medicinal plants have been mostly used for the treatment of wounds (25.3%), cold and influenza (24.6%), stomach (20%), cough (19%), kidney ailments (18.2%), diabetes (13.4%).

Arboflorine, an unusual pentacyclic monoterpenoid indole alkaloid incorporating a third nitrogen atom.

Site Editor — Fri, 19 Jan 2007 18:36:04 -0800

Arboflorine, an unusual pentacyclic monoterpenoid indole alkaloid incorporating a third nitrogen atom.: Org Lett. 2006 Apr 13;8(8):1733-5 Authors: Lim KH, Kam TS

[structure: see text] A new indole alkaloid, arboflorine, possessing a novel pentacyclic carbon skeleton and incorporating a third nitrogen atom was obtained from the Malayan Kopsia arborea. The structure was established by spectroscopic analysis, and a possible biogenetic pathway from a preakuammicine-type precursor is presented.

Ginkgo biloba-an appraisal.

Site Editor — Fri, 19 Jan 2007 18:34:50 -0800

Ginkgo biloba-an appraisal.: Kathmandu Univ Med J (KUMJ). 2004 Jul-Sep;2(3):225-9 Authors: Dubey AK, Shankar PR, Upadhyaya D, Deshpande VY

Ginkgo biloba has been used in traditional Chinese medicine for about 5000 years. A standardized preparation, EGb 761 has been recently prepared. The pharmacologically active constituents, flavonol glycosides and the terpene lactones are standardized. The terpene lactones comprise of ginkgolides A, B, C and bilobalides. The extract scavenges excess free radicals and pretreatment with EGb 761 reduces damage by free radicals in patients undergoing coronary bypass surgery. The action of platelet activating factor is antagonized and platelet aggregation is reduced. Blood flow is increased. Release of prostacyclines and nitric oxide was shown to be stimulated. Ginkgo biloba has been found to be useful in the treatment of Alzheimers disease and cognitive impairment. EGB 761 has shown beneficial effect in aging and mild cognitive impairment. Bilobalide has been shown to be protective against glutamate-induced excitotoxic neuronal death. Early studies indicate a potential role in age-related macular degeneration and some types of glaucoma. Anticancer action is related to antioxidant, anti-angiogenic and gene regulatory actions. Ginkgo biloba has shown overall improvement in about 65% of patients with cerebral impairment and a similar percentage suffering from peripheral vascular diseases. A recent study suggested that phytoestrogens in Ginkgo biloba may have a role as alternative hormone replacement therapy. Recent trials have not shown a beneficial effect of Ginkgo biloba in tinnitus and acute mountain sickness. Ginkgo biloba increased the bioavailability of diltiazem. The extract has been shown to protect against doxorubicin-induced cardiotoxicity and gentamicin-induced nephrotoxicity in animals. Ginkgo biloba inhibits microsomal enzymes and has a potential for drug interactions. Further studies to establish the efficacy of Ginkgo biloba are required.

Protective effects of echinacoside on carbon tetrachloride-induced hepatotoxicity in rats.

Site Editor — Wed, 17 Jan 2007 06:36:52 -0800

Protective effects of echinacoside on carbon tetrachloride-induced hepatotoxicity in rats.: Toxicology. 2006 Dec 23; Authors: Wu Y, Li L, Wen T, Li YQ

The aim of this study was to investigate the possible protective effects of echinacoside, one of the phenylethanoids isolated from the stems of Cistanches salsa, a Chinese herbal medicine, on the free radical damage of liver caused by carbon tetrachloride in rats. Treatment of rats with carbon tetrachloride produced severe liver injury, as demonstrated by dramatic elevation of serum ALT, AST levels and typical histopathological changes including hepatocyte necrosis or apoptosis, haemorrhage, fatty degeneration, etc. In addition, carbon tetrachloride administration caused oxidative stress in rats, as evidenced by increased reactive oxygen species (ROS) production and MDA concentrations in the liver of rats, along with a remarkable reduction in hepatic SOD activity and GSH content. However, simultaneous treatment with echinacoside (50mg/kg, intraperitoneally) significantly attenuated carbon tetrachloride-induced hepatotoxicity. The results showed that serum ALT, AST levels and hepatic MDA content as well as ROS production were reduced dramatically, and hepatic SOD activity and GSH content were restored remarkably by echinacoside administration, as compared to the carbon tetrachloride-treated rats. Moreover, the histopathological damage of liver and the number of apoptotic hepatocytes were also significantly ameliorated by echinacoside treatment. It is therefore suggested that echinacoside can provide a definite protective effect against acute hepatic injury caused by CCl(4) in rats, which may mainly be associated with its antioxidative effect.

[Effectiveness and safety of the treatment of menopausal syndrome with Cimicifuga racemosa dry extract]

Site Editor — Wed, 17 Jan 2007 06:30:11 -0800

[Effectiveness and safety of the treatment of menopausal syndrome with Cimicifuga racemosa dry extract]: Ginekol Pol. 2006 Sep;77(9):678-83 Authors: Radowicki S, Skórzewska K, Rudnicka E, Szlendak-Sauer K, Wierzba W

OBJECTIVES: Phytoestrogens could be an alternative method of the treatment of menopausal syndrome in women with contraindications to hormonal replacement therapy. Design: The aim of the study was to evaluate efficacy and safety of the therapy with Cimicifuga racemosa dry extract. MATERIAL AND METHODS: Twenty women aged mean 52.4 +/- 4.9 years with climacteric syndrome were treated with Cimicifuga racemosa dry extract in a dose of 40 mg a day during 6 months. Kupperman's Index, biochemical parameters and hormonal profile were estimated before and after 3 and 6 months of the therapy. RESULTS: Mean values of Kuppermen's Index were decreased from 30.2 +/- 5.7 points before the therapy to 8.5 +/- 6.3 points after 3 months and to 2.6 +/- 2.1 points after 6 months of the therapy (p < 0.05). No statistical differences in biochemical parameters' concentrations and hormonal profile were observed. CONCLUSIONS: Cimicifuga racemosa dry extract was an effective and safe therapy of climacteric women with contraindications to hormonal replacement therapy.

Inhibition activities of Colchicum luteum baker on lipoxygenase and other enzymes.

Site Editor — Wed, 17 Jan 2007 06:26:01 -0800

Inhibition activities of Colchicum luteum baker on lipoxygenase and other enzymes.: J Enzyme Inhib Med Chem. 2006 Aug;21(4):449-52 Authors: Ahmad B, Khan H, Bashir S, Nisar M, Hassan M

In vitro enzymes inhibition activities of the crude methanolic extract and various fractions of Colchicum luteum Baker (Liliaceae) including chloroform, ethyl acetate, n-butanol and aqueous were carried out against actylcholinesterase, butyrylcholinesterase, lipoxygenase and urease enzymes. A significant enzyme inhibition activity (89%) is shown by the crude methanolic extract and its fractions against lipoxygenase, while low to significant activity (32-75%) was evident against butyrylcholinesterase. The crude methanolic extract and its various fractions demonstrated low activity (29-61%) against acetylcholinesterase and no activity against urease.

Immunomodulatory activity of polysaccharide isolated from Angelica sinensis.

Site Editor — Wed, 17 Jan 2007 06:24:31 -0800

Immunomodulatory activity of polysaccharide isolated from Angelica sinensis.: Int J Biol Macromol. 2006 Nov 15;39(4-5):179-84 Authors: Yang T, Jia M, Meng J, Wu H, Mei Q

The immunomodulatory activities of an Angelica sinensis polysaccharide (AP), purified from the fresh root of A. sinensis Diels, were investigated in vitro in relation to the specificity to immune cells. AP consisted of rhamnose, arabinose, mannose, glucose, galactose with the molar ratio of 1.00:4.54:2.98:11.09:7.45. Cell proliferation results showed that proliferation of total spleen cells, macrophages and T cells were promoted by the action of AP. The treatment of AP increased the production of IL-2 and IFN-gamma, while that of IL-4 was decreased. RT-PCR analysis displayed that the IL-2 and IFN-gamma gene expression were enhanced but the IL-4 gene expression was decreased. Some differences in cytokines secretion pattern were also detected, the expression of IFN-gamma was rapidly augmented while that of IL-2 responded later. The flow cytometry results showed that the percentage of CD4(+)T cell in total spleen cells was remarkably increased by AP, while that of CD8(+)T cell was slightly decreased. In conclusion, AP has immunomodulatory activity by regulating expression of Th1 and Th2 related cytokines. The time-effect relation of cytokines response also suggests that macrophages and natural killer cells involved in nonspecific immunity were primary activated, and helper T cell were secondarily affected by AP.

Conjugating berberine to a multidrug efflux pump inhibitor creates an effective antimicrobial.

Site Editor — Sat, 13 Jan 2007 22:12:55 -0800

The subject of multidrug resistance pumps (MDR) is interesting, particularly when it comes to the comparative MDR activity of the berberine HCL isolate and the apparent synergy inherent in a biochemically-complex extract of Berberis vulgaris. The surprise value of this abstract, however, is the statement "The plant antimicrobial berberine, the active component of the medicinal plants echinacea and golden seal". Holy phytochemistry Batman! Berberine is the active constituent of Echinacea????

Conjugating berberine to a multidrug efflux pump inhibitor creates an effective antimicrobial.: ACS Chem Biol. 2006 Oct 24;1(9):594-600 Authors: Ball AR, Casadei G, Samosorn S, Bremner JB, Ausubel FM, Moy TI, Lewis K

In bacteria, multidrug-resistance pumps (MDRs) confer resistance to chemically unrelated amphipathic toxins. A major challenge in developing efficacious antibiotics is identifying antimicrobial compounds that are not rapidly pumped out of bacterial cells. The plant antimicrobial berberine, the active component of the medicinal plants echinacea and golden seal, is a cation that is readily extruded by bacterial MDRs, thereby rendering it relatively ineffective as a therapeutic agent. However, inhibition of MDR efflux causes a substantial increase in berberine antimicrobial activity, suggesting that berberine and potentially many other compounds could be more efficacious if an effective MDR pump inhibitor could be identified. Here we show that covalently linking berberine to INF 55 , an inhibitor of Major Facilitator MDRs, results in a highly effective antimicrobial that readily accumulates in bacteria. The hybrid molecule showed good efficacy in a Caenorhabditis elegans model of enterococcal infection, curing worms of the pathogen.

Devil's Claw (Harpagophytum procumbens) as a Treatment for Osteoarthritis: A Review of Efficacy and Safety.

Site Editor — Sat, 13 Jan 2007 21:37:09 -0800

Devil's Claw (Harpagophytum procumbens) as a Treatment for Osteoarthritis: A Review of Efficacy and Safety.: J Altern Complement Med. 2006 Dec; 12(10): 981-93. Authors: Brien S, Lewith GT, McGregor G

Background: Osteoarthritis (OA) is a highly prevalent musculoskeletal disorder. Conventional treatment (i.e., the use of nonsteroidal anti-inflammatory drugs-NSAIDs) is associated with well-documented adverse effects. Devil's Claw (Harpagophytum procumbens) a traditional South African herbal remedy used for rheumatic conditions, may be a safer treatment option. To date, 14 clinical trials have assessed its efficacy/ effectiveness in OA. Aim: To address the two main questions of importance to clinicians: (1) Does Devil's Claw work for the treatment of OA, and (2) Is it safe? Methods: A review of the literature on Devil's Claw and OA from 1966 to 2006 was performed using multiple search databases, monographs, and citation tracking. Relevant trials in all languages were identified and included. Both internal validity (i.e., adequacy of the dosage and period of treatment for this condition, reporting of randomization, rates of dropout, blinding, and statistical analysis) and external validity (i.e., inclusion/ exclusion criteria, baseline characteristics of the study populations, trial setting, and the appropriateness of the outcome measures of the trials) were assessed. Results: Fourteen studies were identified: eight observational studies; 2 comparator trials (1 open, the other randomized to assess clinical effectiveness); and 4 double-blinded, placebo-controlled, randomized controlled trials to assess efficacy. Many of the published trials lacked certain important methodological quality criteria. However, the data from the higher quality studies suggest that Devil's Claw appeared effective in the reduction of the main clinical symptom of pain. The assessment of safety is limited by the small populations generally evaluated in the clinical studies. From the current data, Devil's Claw appears to be associated with minor risk (relative to NSAIDs), but further long-term assessment is required. Conclusions: The methodological quality of the existing clinical trials is generally poor, and although they provide some support, there are a considerable number of methodologic caveats that make further clinical investigations warranted. The clinical evidence to date cannot provide a definitive answer to the two questions posed: (1) Does it work? And (2) is it safe? A definitive high-quality trial that addresses the necessary methodologic improvements noted is needed to answer these important clinical questions.

Trial of Essiac to Ascertain Its Effect in Women with Breast Cancer (TEA-BC).

Site Editor — Sat, 13 Jan 2007 21:36:04 -0800

Trial of Essiac to Ascertain Its Effect in Women with Breast Cancer (TEA-BC).: J Altern Complement Med. 2006 Dec; 12(10): 971-80. Authors: Zick SM, Sen A, Feng Y, Green J, Olatunde S, Boon H

Background: Breast cancer is a major cause of morbidity, mortality, and medical expenditures among women in Canada. Essiac (Resperintrade mark Canada Limited, Waterloo, Ontario, Canada), a blend of at least four herbs (burdock root [Arctium lappa], Indian rhubarb [Rheum palmatum], sheep sorrel [Rumex acetosella], and the inner bark of slippery elm [Ulmus fulva or U. rubra]), has become one of the more popular herbal remedies for breast-cancer treatment, secondary prevention, improving quality of life, and controlling negative side-effects of conventional breast-cancer treatment. Objectives: Our primary objective was to determine the difference in health-related quality of life (HR-QOL), as assessed by the Functional Assessment of Cancer Therapy Breast Cancer Version, between women who are new Essiac users (since breast cancer diagnosis) and those who have never used Essiac. Secondary endpoints included differences in depression, anxiety, fatigue, rate of adverse events, and prevalence of complications or benefits associated with Essiac during standard breast-cancer treatment. Additionally, we described the pattern of use of Essiac in this cohort of women. Methods: We performed a retrospective cohort study in 510 women, randomly chosen from the Ontario Cancer Tumour Registry, with a diagnosis of primary breast cancer in 2003. Results: With the exception changes in a Physical well-being subscale and a relationship with doctor subscale, Essiac did not have a significant effect on HR-QOL or mood states. Even for Physical well-being and relationship with doctor, Essiac seemed to have a negative effect, with Essiac users doing worse than the non-Essiac users. This might be attributed to the fact that the group of users comprised younger women with more advanced stages of breast cancer, and both of these subgroups of patients have been shown to be at a significantly increased risk for negative mood states and/or a decreased sense of well-being. The women were taking low doses (total daily dose 43.6 +/- 30.8 mL) of Essiac that corresponded to the label directions found on most Essiac products. Friends were the most common source of information, and most women were taking Essiac to boost their immune systems or increase their chances of survival. Only 2 women reported minor adverse events, whereas numerous women reported beneficial effects of Essiac. Conclusions: Essiac does not appear to improve HR-QOL or mood states. Future studies are needed to determine whether other clinical outcomes, such as cancer reoccurrence, are affected by Essiac.

Hepatoprotective herbal drug, silymarin from experimental pharmacology to clinical medicine.

Site Editor — Sat, 13 Jan 2007 21:35:06 -0800

Hepatoprotective herbal drug, silymarin from experimental pharmacology to clinical medicine.: Indian J Med Res. 2006 Nov; 124(5): 491-504. Authors: Pradhan SC, Girish C

Silymarin, a flavonolignan from 'milk thistle' (Silybum marianum) plant is used almost exclusively for hepatoprotection and amounts to 180 million US dollars business in Germany alone. In this review we discuss about its safety, efficacy and future uses in liver diseases. The use of silymarin may replace the polyherbal formulations and will avoid the major problems of standardization, quality control and contamination with heavy metals or bacterial toxins. Silymarin consists of four flavonolignan isomers namely- silybin, isosilybin, silydianin and silychristin. Among them, silybin being the most active and commonly used. Silymarin is orally absorbed and is excreted mainly through bile as sulphates and conjugates. Silymarin offers good protection in various toxic models of experimental liver diseases in laboratory animals. It acts by antioxidative, anti-lipid peroxidative, antifibrotic, anti-inflammatory, membrane stabilizing, immunomodulatory and liver regenerating mechanisms. Silymarin has clinical applications in alcoholic liver diseases, liver cirrhosis, Amanita mushroom poisoning, viral hepatitis, toxic and drug induced liver diseases and in diabetic patients. Though silymarin does not have antiviral properties against hepatitis virus, it promotes protein synthesis, helps in regenerating liver tissue, controls inflammation, enhances glucuronidation and protects against glutathione depletion. Silymarin may prove to be a useful drug for hepatoprotection in hepatobiliary diseases and in hepatotoxicity due to drugs. The non traditional use of silymarin may make a breakthrough as a new approach to protect other organs in addition to liver. As it is having a good safety profile, better patient tolerability and an effective drug at an affordable price, in near future new derivatives or new combinations of this drug may prove to be useful.

The Induction of CYP1A2, CYP2D6 and CYP3A4 by Six Trade Herbal Products in Cultured Primary Human Hepatocytes.

Site Editor — Sat, 13 Jan 2007 21:34:04 -0800

The Induction of CYP1A2, CYP2D6 and CYP3A4 by Six Trade Herbal Products in Cultured Primary Human Hepatocytes.: Basic Clin Pharmacol Toxicol. 2007 Jan; 100(1): 23-30 Authors: Hellum BH, Hu Z, Nilsen OG

The aim of this study was to evaluate the in vitro inductive potential of six commonly used trade herbal products on CYP1A2, CYP2D6 and CYP3A4 metabolic activities. Herbal components were extracted from the trade products in a way that ensured a composition equal to that present in the original product. Primary human hepatocytes and specific CYP substrates were used. Classic inducers were used as positive controls and herbal extracts were added in in vivo-relevant concentrations. Metabolites were determined by high performance liquid chromatography (HPLC). St. John's wort and common valerian were the strongest inducing herbs. In addition to induction of CYP3A4 by St. John's wort, common valerian and Ginkgo biloba increased the activity of CYP3A4 and 2D6 and CYP1A2 and 2D6, respectively. A general inhibitory potential was observed for horse chestnut, Echinacea purpurea and common sage. St. John's wort inhibited CYP3A4 metabolism at the highest applied concentration. Horse chestnut might be a herb with high inhibition potentials in vivo and should be explored further at lower concentrations. We show for the first time that G. biloba may exert opposite and biphasic effects on CYP1A2 and CYP2D6 metabolism. Induction of CYP1A2 and inhibition of CYP2D6 were found at low concentrations; the opposite was observed at high concentrations. CYP2D6 activity, regarded generally as non-inducible, was increased by exposure to common valerian (linear to dose) and G. biloba (highest concentration). An allosteric activation is suggested. From the data obtained, G. biloba, common valerian and St. John's wort are suggested as candidates for clinically significant CYP interactions in vivo.

Sunflower therapy for children with specific learning difficulties (dyslexia): A randomised, controlled trial.

Site Editor — Sat, 13 Jan 2007 21:32:13 -0800

Sunflower therapy for children with specific learning difficulties (dyslexia): A randomised, controlled trial.: Complement Ther Clin Pract. 2007 Feb; 13(1): 15-24 Bull L

The aim of the study was to determine the clinical and perceived effectiveness of the Sunflower therapy in the treatment of childhood dyslexia. The Sunflower therapy includes applied kinesiology, physical manipulation, massage, homeopathy, herbal remedies and neuro-linguistic programming. A multi-centred, randomised controlled trial was undertaken with 70 dyslexic children aged 6-13 years. The research study aimed to test the research hypothesis that dyslexic children 'feel better' and 'perform better' as a result of treatment by the Sunflower therapy. Children in the treatment group and the control group were assessed using a battery of standardised cognitive, Literacy and self-esteem tests before and after the intervention. Parents of children in the treatment group gave feedback on their experience of the Sunflower therapy. Test scores were compared using the Mann Whitney, and Wilcoxon statistical tests. While both groups of children improved in some of their test scores over time, there were no statistically significant improvements in cognitive or Literacy test performance associated with the treatment. However, there were statistically significant improvements in academic self-esteem, and reading self-esteem, for the treatment group. The majority of parents (57.13%) felt that the Sunflower therapy was effective in the treatment of learning difficulties. Further research is required to verify these findings, and should include a control group receiving a dummy treatment to exclude placebo effects.

Efficacy of an herbal dietary supplement (Smooth Move) in the management of constipation in nursing home residents...

Site Editor — Wed, 10 Jan 2007 21:44:48 -0800

Efficacy of an herbal dietary supplement (Smooth Move) in the management of constipation in nursing home residents: A randomized, double-blind, placebo-controlled study.: J Am Med Dir Assoc. 2006 Nov;7(9):556-61 Authors: Bub S, Brinckmann J, Cicconetti G, Valentine B

OBJECTIVE: To investigate the efficacy and cost effectiveness of an herbal tea, Smooth Move, in nursing home residents with chronic constipation. DESIGN: Double-blind, placebo-controlled, 2-armed, parallel-group clinical trial. SETTING: A 483-bed nursing home in Allentown, Pennsylvania, operated by Lehigh County Government. PARTICIPANTS: A total of 86 nursing home residents with chronic constipation. INTERVENTIONS: Participants (n = 86) were randomly assigned to receive Smooth Move (n = 42) or a placebo (n = 44), once daily, in addition to standard treatment for chronic constipation. The study period was 28 days. MEASUREMENTS: The primary efficacy parameter was the difference in total number of bowel movements. Secondary parameters included the difference in average number of standard treatment doses dispensed, and the difference in total medication costs. RESULTS: Compared to placebo, in the intention to treat (ITT analysis) there was a statistically significant increase in the number of bowel movements in the Smooth Move group. The Smooth Move group (n = 42) compared with the placebo group (n = 44) experienced an average of 4.14 more bowel movements during the 28-day study period versus the 28-day pre-study period (P = .017). CONCLUSION: Smooth Move herbal tea, when added to the standard treatment regimen for nursing home residents with chronic constipation, increased the average number of bowel movements compared to the addition of a placebo tea.

Mac OSX widget for Herbal Science Research feed

Site Editor — Wed, 10 Jan 2007 20:59:48 -0800

A shiny new Mac OSX widget is now available for the Herbal Science Research feed. This widget pulls in a feed of the latest posts from HerbalScienceResearch.com, including PubMed abstracts from NLM filtered using a complex custom MeSH string created for this site.

Now for the disclaimer...this is an introductory effort and should be used with caution. I am running it on 10.4.8 and have seen no problems with it so far.

I do plan to add a pull-down menu to allow you to subscribe to any of the feeds coming through the site and/or specific PubMed category feeds. Meanwhile, enjoy having the very latest Herbal Science Research feed as close as your Mac's Dashboard.

A windows version will be coming at some point in the near future.

Download the widget here.

Once downloaded, unzip the widget by double-clicking on the HSR.zip file. Then, double-click the widget to install it. Easy as Apple pie.

Enjoy.

Green tea polyphenol stimulates cancer preventive effects of celecoxib in human lung cancer cells...

Site Editor — Tue, 09 Jan 2007 06:31:59 -0800

Green tea polyphenol stimulates cancer preventive effects of celecoxib in human lung cancer cells by upregulation of GADD153 gene.: Int J Cancer. 2006 Jul 1;119(1):33-40 Authors: Suganuma M, Kurusu M, Suzuki K, Tasaki E, Fujiki H

To more clearly understand the molecular mechanisms involved in synergistic enhancement of cancer preventive activity with the green tea polyphenol (-)-epigallocatechin gallate (EGCG), we examined the effects of cotreatment with EGCG plus celecoxib, a cyclooxygenase-2 selective inhibitor. We specifically looked for induction of apoptosis and expression of apoptosis related genes, with emphasis on growth arrest and DNA damage-inducible 153 (GADD153) gene, in human lung cancer cell line PC-9: Cotreatment with EGCG plus celecoxib strongly induced the expression of both GADD153 mRNA level and protein in PC-9 cells, while neither EGCG nor celecoxib alone did. However, cotreatment did not induce expression of other apoptosis related genes, p21(WAF1) and GADD45. Judging by upregulation of GADD153, only cotreatment with EGCG plus celecoxib synergistically induced apoptosis of PC-9 cells. Synergistic effects with the combination were also observed in 2 other lung cancer cell lines, A549 and ChaGo K-1. Furthermore, EGCG did not enhance GADD153 gene expression or apoptosis induction in PC-9 cells in combination with N-(4-hydroxyphenyl)retinamide or with aspirin. Thus, upregulation of GADD153 is closely correlated with synergistic enhancement of apoptosis with EGCG. Cotreatment also activated the mitogen-activated protein kinases (MAPKs), such as ERK1/2 and p38 MAPK: Preteatment with PD98059 (ERK1/2 inhibitor) and UO126 (selective MEK inhibitor) abrogated both upregulation of GADD153 and synergistic induction of apoptosis of PC-9 cells, while SB203580 (p38 MAPK inhibitor) did not do so, indicating that GADD153 expression was mediated through the ERK signaling pathway. These findings indicate that high upregulation of GADD153 is a key requirement for cancer prevention in combination with EGCG.

How to Design a Phase I Trial of an Anticancer Botanical

Site Editor — Tue, 09 Jan 2007 06:26:31 -0800

How to Design a Phase I Trial of an Anticancer Botanical. J Soc Integr Oncol 2006;4(1):46–51 Authors: Vickers Andrew J.

Phase I trials are an important part of traditional drug development in oncology. Such trials address two key issues: safety and dose. Currently, there is a dearth of phase I trials of anticancer botanicals. This may result from the apparently widespread view that a history of human use precludes the need for early-phase study. However, the safe use of a botanical by the population at large does not guarantee safety when the botanical is used in combination with other agents in the complex medical setting of oncology. Several cases of unpredictable adverse events have been recorded following the use of botanicals by cancer patients.We propose a simple, robust design for phase I trials of anticancer botanicals. This design incorporates important characteristics of botanical medicines including low toxicity, prior data on a likely safe dose, a limit on the highest dose it is feasible to administer, and the unknown relationship between dose-toxicity and dose-response curves. Two principal design features are the use of predetermined dose levels and the direct measurement of a response endpoint such as survival or immunity. This response end point can be used to determine the optimal dose if toxicity is acceptable at all dose levels.Increasing the use of phase I methodology would ensure a more systematic development of botanicals as anticancer agents. This would likely increase the chance that at least one such agent would be proven to extend lives.

In vitro antibacterial activity of some plant essential oils.

Site Editor — Tue, 09 Jan 2007 06:09:51 -0800

In vitro antibacterial activity of some plant essential oils.:
BMC Complement Altern Med. 2006;6:39 Authors: Prabuseenivasan S, Jayakumar M, Ignacimuthu S

BACKGROUND: To evaluate the antibacterial activity of 21 plant essential oils against six bacterial species. METHODS: The selected essential oils were screened against four gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus vulgaris) and two gram-positive bacteria Bacillus subtilis and Staphylococcus aureus at four different concentrations (1:1, 1:5, 1:10 and 1:20) using disc diffusion method. The MIC of the active essential oils were tested using two fold agar dilution method at concentrations ranging from 0.2 to 25.6 mg/ml. RESULTS: Out of 21 essential oils tested, 19 oils showed antibacterial activity against one or more strains. Cinnamon, clove, geranium, lemon, lime, orange and rosemary oils exhibited significant inhibitory effect. Cinnamon oil showed promising inhibitory activity even at low concentration, whereas aniseed, eucalyptus and camphor oils were least active against the tested bacteria. In general, B. subtilis was the most susceptible. On the other hand, K. pneumoniae exhibited low degree of sensitivity. CONCLUSION: Majority of the oils showed antibacterial activity against the tested strains. However Cinnamon, clove and lime oils were found to be inhibiting both gram-positive and gram-negative bacteria. Cinnamon oil can be a good source of antibacterial agents.

[Pharmacological effects of flavonoids from Scutellaria baicalensis]

Site Editor — Tue, 09 Jan 2007 06:08:15 -0800

[Pharmacological effects of flavonoids from Scutellaria baicalensis]: Przegl Lek. 2006;63(2):95-6 Authors: Kowalczyk E, Krzesiński P, Kura M, Niedworok J, Kowalski J, Błaszczyk J

Scutellaria baicalensis is a plant widely used in Chinese and Japanese herbal medicine. Dry roots of Scutellaria baicalensis are used, especially as alcohol extracts. Flavonoids isolated from Radix Scutellariae have beneficial effects in hepatitis. Flavonoids derived from Scutellaria baicalensis produce antioxidative, antineoplastic, cardiomiocyte-protective activity. They inhibit agregation of platelets, permeability of capillary vessels, have antibacterial and anty-angiogenic effects.

Natural Products: From Structures to Mechanisms and Potential For Therapeutics

Site Editor — Mon, 08 Jan 2007 22:47:18 -0800

Natural Products: From Structures to Mechanisms and Potential For Therapeutics: Presented by: Carole Bewley, Ph.D., NIDDK

NIH Director's Seminars | Aired date: 2/17/2006 4:15:00 PM Eastern Time

The effects of tea extracts on proinflammatory signaling.

Site Editor — Sat, 06 Jan 2007 19:02:59 -0800

The effects of tea extracts on proinflammatory signaling.: BMC Med. 2006;4:28 Authors: Pajonk F, Riedisser A, Henke M, McBride WH, Fiebich B

BACKGROUND: Skin toxicity is a common side effect of radiotherapy for solid tumors. Its management can cause treatment gaps and thus can impair cancer treatment. At present, in many countries no standard recommendation for treatment of skin during radiotherapy exists. In this study, we explored the effect of topically-applied tea extracts on the duration of radiation-induced skin toxicity. We investigated the underlying molecular mechanisms and compared effects of tea extracts with the effects of epigallocatechin-gallate, the proposed most-active moiety of green tea. METHODS: Data from 60 patients with cancer of the head and neck or pelvic region topically treated with green or black tea extracts were analyzed retrospectively. Tea extracts were compared for their ability to modulate IL-1beta, IL-6, IL-8, TNFalpha and PGE2 release from human monocytes. Effects of tea extracts on 26S proteasome function were assessed. NF-kappaB activity was monitored by EMSAs. Viability and radiation response of macrophages after exposure to tea extracts was measured by MTT assays. RESULTS: Tea extracts supported the restitution of skin integrity. Tea extracts inhibited proteasome function and suppressed cytokine release. NF-kappaB activity was altered by tea extracts in a complex, caspase-dependent manner, which differed from the effects of epigallocatechin-gallate. Additionally, both tea extracts, as well as epigallocatechin-gallate, slightly protected macrophages from ionizing radiation CONCLUSION: Tea extracts are an efficient, broadly available treatment option for patients suffering from acute radiation-induced skin toxicity. The molecular mechanisms underlying the beneficial effects are complex, and most likely not exclusively dependent on effects of tea polyphenols such as epigallocatechin-gallate.

A retrospective case-control study of the use of hormone-related supplements and association with breast cancer.

Site Editor — Sat, 06 Jan 2007 19:01:39 -0800

A retrospective case-control study of the use of hormone-related supplements and association with breast cancer.: Int J Cancer. 2007 Jan 4; Authors: Rebbeck TR, Troxel AB, Norman S, Bunin GR, Demichele A, Baumgarten M, Berlin M, Schinnar R, Strom BL

Hormone-related supplements (HRS), many of which contain phytoestrogens, are widely used to manage menopausal symptoms, yet their relationship with breast cancer risk has generally not been evaluated. We evaluated whether use of HRS was associated with breast cancer risk, using a population-based case-control study in 3 counties of the Philadelphia metropolitan area consisting of 949 breast cancer cases and 1,524 controls. Use of HRS varied significantly by race, with African American women being more likely than European American women to use any herbal preparation (19.2% vs. 14.7%, p = 0.003) as well as specific preparations including black cohosh (5.4% vs. 2.0%, p = 0.003), ginseng (12.5% vs. 7.9%, p < 0.001) and red clover (4.7% vs. 0.6%, p < 0.001). Use of black cohosh had a significant breast cancer protective effect (adjusted odds ratio 0.39, 95% CI: 0.22-0.70). This association was similar among women who reported use of either black cohosh or Remifemin (an herbal preparation derived from black cohosh; adjusted odds ratio 0.47, 95% CI: 0.27-0.82). The literature reports that black cohosh may be effective in treating menopausal symptoms, and has antiestrogenic, antiproliferative and antioxidant properties. Additional confirmatory studies are required to determine whether black cohosh could be used to prevent breast cancer. (c) 2006 Wiley-Liss, Inc.